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AGARCas No. 009002 18 0 Technical Specifics Sieve analysis (ASTM Retained on Mesh 80) Max 5% Gel strength >900 and <1050 g cm pH (1. 5 % solution) (value not specified) Gel after autoclave 6 7. 5 Loss on drying <5 % Melting point (C) >85 C Gelling point (C) 34 36 C Store dry at room temperature Agar used for microbiology is derived from algae and is valued for its inert nature. It forms solid and semisolid media essential for isolating microorganisms and

Component g/l Peptone 14 Yeast Extract 7 KH2PO4 0

31 pH (100 g/l) 4

Synthetic Defined Minimal Media - FM Minimal medium w/o Amino acids and w/o Ammonium chloride

Tryptone 8 Yeast extract 5 NaCl 5 Agar 15 Final pH 7

0 Beef Extract 5

5 Purity HPLC >99% Appearance White Powder Store dry at room temperature

Unlike glucose

Weight 165

Cold Spring harbour

Suspend 52

Yeast extract 10 Peptone 20 Galactose 20 Agar 20 Suspend 70 gram powdered medium in 1 litre distilled water

0 Yeast Extract 2